|Abstract Title:||Understanding phosphorylation-mediated effects on NF-κB interactions using IM-MS|
|Session Choice:||Ion Mobility - Mass Spectrometry (BMSS)|
|Presenter Name:||Prof Claire Eyers|
|Company/Organisation:||University of Liverpool|
Abstract Information :
The NF-κB system is a critical signalling pathway mediating (amongst other things) cellular responses to inflammation and DNA damage. The five NF-κB family members are transcription factors that are extensively regulated by phosphorylation, which regulates its ability to dimerise and to bind DNA, although the precise regulatory mechanisms of many of these sites remain to be elucidated. Using a combination of 'bottom-up' phosphosite mapping and 'native' ion mobility mass spectrometry, we can start to delineate the roles of individual sites of phosphorylation on NF-κB interactions, stability and dynamics.