HTC-15 - Abstract

Abstract Title: UHPSFC-MS of a Range of Steroidal Compounds
Abstract Type: Tutorial
Session Choice: Green Separations
Presenter Name: Ms Julie Herniman
Co-authors:Prof John Langley
Company/Organisation: University of Southampton
Country: United Kingdom

Abstract Information :

Steroids are a category of lipid molecules that include cholesterol, steroid hormones and bile salts. Traditionally gas chromatography mass spectrometry (GC-MS) has been used for analysis but often requires derivatisation to increase the volatility of the samples. Reversed phase HPLC-MS has been used for the analysis of many steroid compounds, however, some are over-retained and/or separation of mixtures is difficult to achieve. More recently high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been utilised due to its improved specificity and selectivity. Similar to the GC approaches, derivatisation of the steroid is often required to aid ionisation.

There are varied research interests in lipids at Southampton and the application of UHPSFC-MS continues to grow in this field, and has recently been extended to a different steroidal compound classes. A Waters Acquity UPC2, coupled to a TQD mass spectrometer and different organic make-up solvents are used to promote ionisation for a range of different ionisation techniques, namely ESI, APCI and APPI. Separation was developed for mixtures of steroid compounds, such as cholanic acids/esters and steroid derivatives using different column phases and different modifier solvent gradients. The methods used for standard compounds have subsequently been embedded into an open access environment to facilitate the analysis of archaeological materials, biofuels and to monitor chemical synthesis.