|Sequential Three-Dimensional Gas Chromatography with Accurate Mass Spectrometry: A Novel Tool for High-Resolution Characterization of Multicomponent Samples
|Comprehensive Chromatography - The State of the Art
|Ms Dandan Yan
|Unversity of Tasmania
Abstract Information :
Innovations on the theme of multidimensional gas chromatography (MDGC) and comprehensive two-dimensional GC (GCxGC) separations, with the aim of expanding peak capacity, are of continuing interest in chromatography. This presentation will highlight our work on the development of an integrated multidimensional three-dimensional gas chromatography (3D-GC) approach for the study of secondary metabolite compounds in essential oils. This integrated system incorporates a non-polar first-dimension (1Dnp) separation step, prior to microfluidic Deans switch heart-cutting of a targeted region(s) into a polar second-dimension (2DPEG) column for multidimensional separation (GC-GC). If further separation is required, the effluent from 2DPEG may be modulated to produce a GCxGC separation, using an ionic liquid phase as 3D column (3DIL). In practice, it operates with preliminary 1D separation of a complex matrix using GC-FID, followed by extraction of target regions into a second (GC-GC) and/or a third column (GC-GCxGC) for additional successive separations; i.e. it operates as a sequential GCnp-GCPEGxGCIL. The analytical benefits of employing three varied chemical selectivities in the 3D separation, coupled with accurate mass time-of-flight mass spectrometric detection will be discussed. The described system may be used in a number of modes, but one useful approach is to target specific classes of compounds for improved resolution. This is demonstrated here through the separation and detection of oxygenated sesquiterpenes in hop (Humulus lupulus L.) and agarwood (Aquilaria malaccensis) essential oils.