|Separation and characterisation by ion mobility-mass spectrometry
|Separations by Ion Mobility
|Dr Antonio Calabrese
|University of Leeds
Abstract Information :
Ion mobility spectrometry involves the separation of ions in the gas phase based on their mass, collision cross-section (size, shape) and charge. Whilst ion mobility has found use as a standalone technique (for example in the detection of explosives and narcotics), it is often combined with mass spectrometry and high-performance liquid chromatography. Ion mobility can be used to separate analytes from the sample background, separate charge states and resolve isobaric ions. Moreover, collision cross-sections can be used to validate analyte identifications, or provide structural information for both small and large molecules and complexes. In particular, ion mobility-mass spectrometry has emerged as an invaluable tool in the structural biology toolkit. Several ion mobility devices (e.g. drift tube, travelling wave, FAIMS and TIMS) have been developed and commercialized. In this presentation, the theory of ion mobility, commercially available devices, and several applications will be highlighted, demonstrating the utility of ion mobility-mass spectrometry in a range of Omics fields.