HTC-15

HTC-15 - Abstract

Abstract Title: Recent advances in the analysis of protein biopharmaceuticals
Abstract Type: Seminar
Presenter Name: Koen Sandra
Co-authors: Jonathan Vandenbussche
Isabel Vandenheede
Mieke Steenbeke
Emmie Dumont
Gerd Vanhoenacker
Pat Sandra
Company/Organisation: Research Institute for Chromatography
Session Choice: (R)evolutions in Biopharmaceutical Analysis (KVCV)

Abstract Information :

Protein therapeutics are becoming a core aspect of the pharmaceutical industry. Together with a huge therapeutic potential, these molecules come with a structural complexity that drives state-of-the-art chromatography and mass spectrometry to its limits. The present contribution reports on recent advances in the analysis of protein biopharmaceuticals at protein, peptide and glycan level. The versatility of hydrophilic interaction chromatography (HILIC), the benefits of instrument and column inertness, the power of comprehensive two-dimensional liquid chromatography (LCxLC) and the possibilities of micropillar array columns (µPAC), all in hyphenation to high-resolution mass spectrometry (MS), will be discussed and exemplified using therapeutic enzymes, monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs).