|Quantification of the extended list of suspected allergens in fragrance materials by GCxGC-TOFMS
|Exploiting Separation Science
|Dr Sebastiano Panto
|Mr Alan Griffith
|LECO European Application & Technology Center
Abstract Information :
In 2011, the Scientific Committee on Consumer Safety (SCCS) proposed to extend the list of 24 suspected allergens to 57 compounds, including all the isomeric forms. As a consequence, a new legislation is expected in the next future regarding the information to be provided to consumers. The impact for the companies involved in this field will be high; they need to adapt/modify their analytical methods in order to accomplish the challenge of quantify the updated list of substances.
The method currently applied for the 24 allergens is based on two parallel GC-MS separations with two different stationary phases (apolar and mid-polar stationary phases). This approach usually overcomes coelutions of the current suspected allergens with matrix. However this becomes more difficult when considering the new list of allergens, especially if isomers, allergen precursors and additional regulated substances are considered. In order to overcome these issues, especially with most complex matrices, a comprehensive two-dimensional gas chromatography approach (GCxGC-TOFMS) has been developed. Different parameters have been tested in order to reach a satisfactory separation between the target compounds. Finally, an apolar x mid-polar column set has been exploited for the development of the GCxGC method.
The method has been successfully applied to different commercial fragrances and their respective commercial copy products.