|Opportunities for ultra-rapid LC-MS/MS in high-throughput bioanalysis
|Advances in Clinical Analysis
|Dr Lewis Couchman
|Analytical Services International (ASI) Ltd.
Abstract Information :
Typically, targetted quantitative LC-MS/MS analyses using gradient elution are carried out at the rate of a few minutes per injection. In this paper, an approach for ultra-rapid LC-MS/MS analysis will be described in which complete injection-to-injection cycle-times are just 30 seconds, equating to at least one 96-well plate per hour. These cycle times are more akin to those of direct injection methods such as flow-injection analysis, but despite this include efficient chromatographic separation of target analytes. This enables
(i) the removal of interference from isobaric compounds (e.g. from other drugs and/or metabolites) and also
(ii) the reduction of ionization effects caused by co-eluting matrix components. In this presentation, application of the approach will be demonstrated using examples from therapeutic drug monitoring and clinical and forensic toxicology. The practical considerations for implementation of the approach to dramatically speed up other analyses will be discussed.