|Abstract Title:||Online extraction and determination of carotenoids from food sample by means of supercritical fluid extraction-supercritical fluid chromatography-mass spectrometry|
|Session Choice:||Green Separations|
|Presenter Name:||Dr Mariosimone Zoccali|
|Co-authors:||Prof Daniele Giuffrida|
Dr Adriana Arigò
Prof Paola Dugo
Prof Luigi Mondello
|Company/Organisation:||University of Messina|
Abstract Information :
Extraction and detection of bioactive compounds from food samples is a very hard task. Conventional extraction procedures are usually time consuming and can require a very high amount of organic solvents. Moreover bioactive compounds can be photolabile and/or thermolabile, in this cases must be immediately injected to avoid any kind of degradation.
The aim of this research was focused on the development of an online method coupling supercritical fluid extraction and supercritical fluid chromatography (SFC) for the extraction and detection of carotenoids from Tamarillo samples. The online nature of the system, compared to offline approaches, improves run-to-run precision, enables the setting of batch-type applications, and reduces the risks of sample loss and contamination.
Carotenoids were extracted and detected in less than 17 minutes by using few mL of methanol, including free carotenoids, carotenoids monoesters and carotenoids diesters, in a very fast, and efficient way. Multiple extractions, until depletion, were performed on the same sample, in order to evaluate the extraction yield and to obtain quantitative data. The online supercritical fluid extraction supercritical fluid chromatography method developed was then compared with the traditional solid-liquid extraction HPLC analysis giving comparable results. The proposed methodology developed in this work provides a new platform for the online extraction and separation of relatively nonpolar compounds, showing the capability of this methodology to greatly reduce the analytical times and solvent consuming in a very fast and efficient way.