HTC-15 - Abstract

Abstract Title: LC-MS small molecule quantitation: a short tutorial of best practice
Abstract Type: Tutorial
Session Choice: Challenges in Quantitative Analysis
Presenter Name: Dr Ruth Godfrey
Company/Organisation: Swansea University
Country: United Kingdom

Abstract Information :

Advancement of liquid chromatography-mass spectrometry (LC-MS) instrumentation, operating systems and software has enabled small molecule quantitation by this technique to become part of routine practice. However, many challenges remain in ensuring reliable and accurate quantitation, particularly when analysing complex samples and using soft ionization approaches, such as electrospray ionization. The solution(s) to these challenges is sometimes subtle and not easily accessible, often resulting in them being overlooked by non-expert users. A role of the Analytical Methods Committee (AMC) of the Royal Society of Chemistry (RSC) is to engage with both expert and non-expert users, providing educational and guidance material to enhance and support understanding for using analytical techniques. This tutorial is inspired by guidance prepared on behalf of AMC designed to help inform and support the use of LC-MS for small molecule quantitation.