|Interfacing LC to MS
|Ms Julie Herniman
|University of Southampton
Abstract Information :
Whilst interfacing of liquid chromatography to mass spectrometry is now commonplace there are a number of important factors that need to be considered when using modern LC-MS. If approaching LC-MS from a chromatography background then compatibility of buffers used for reversed phase LC must be considered prior to the use of mass spectrometry as the detector.
Coupling of normal phase chromatography to mass spectrometry is inherently flawed, e.g. due to lack of protic media for electrospray ionisation; SFC-MS will be discussed as a surrogate for normal phase chromatography coupled to mass spectrometry.
This tutorial will explore the different approaches required to enable successful transfer of LC methods to LC-MS methods and also focus on different atmospheric pressure ionisation sources and optimisation thereof.