HTC-15 - Abstract

Abstract Title: Green Bioanalytical Analysis of Voriconazole and Tadalafil by HPLC
Abstract Type: Seminar
Session Choice: Green Separations
Presenter Name: Dr Aysegul Dogan
Co-authors:Prof Nursabah E. Basci
Company/Organisation: Hacettepe University Faculty of Pharmacy Department of Analytical Chemistry
Country: Turkey

Abstract Information :

Green chromatography techniques are a developing area in the scientific research studies due to their security for environment and human health. Greening procedures in HPLC techniques may be based on reduction of toxic solvent usage and/or the replacement of toxic solvents with less or non-toxic ones which were friendly to the environment. In this study; 30 drug molecules having different physicochemical properties analyzed using firstly with conventional organic modifiers, then with propylene carbonate included mobile phases and three of the molecules with the best chromatographic separation were selected for analyzing from plasma. Green HPLC methods of voriconazole, tadalafil and phenazopyridine (internal standart) was developed and applied in the light of model molecules, voriconazole and tadalafil, to the analysis from spiked plasma samples. For this purpose, non-toxic propylene carbonate and ethanol was used as solvents, and new generation low particle sized small column and microflow high performance liquid chromatography technique approaches was also tested. Shim-Pack XR-ODS (100 x 2.0 mm, 2.2 µm) analytical column was utilized for the analysis of voriconazole and tadalafil by using phenazoprydine as internal standart, and diode array dedector was set to 256 nm for voriconazole and 284 nm for tadalafil. Mobile phase composition was constracted as PC/(% 70 phosphate buffer (50 mM, pH: 3.0) + % 30 EtOH) (10:90, v/v) at a flow rate 0.3 mL min-1. Developed analysis methods were validated in terms of stability, linearity, accuracy, precision, specificity, ruggedness and robustness. Developed and optimized methods were tested for their applicability and relaibility plasma analysis by analytical/bioanalytical method validation strategies and according to the findings the methods were found to be accurate, precise, specific, sensitive, rugged, robust and reliabile in long term use.