|Abstract Title:||Combined separation strategy for chiral method development of acidic and non-acidic pharmaceutical compounds in capillary electrochromatography (CEC) separation technique|
|Session Choice:||Comprehensive Chromatography - The State of the Art|
|Presenter Name:||Dr Dima Albals|
|Co-authors:||Dr Ans Hendrickx|
Prof Debby Mangelings
Prof Yvan Vander Heyden
|Company/Organisation:||Faculty of pharmacy, Department of Pharmaceutical science, Yarmouk University, Irbid, Jordan.|
Abstract Information :
Chiral drug molecules consist of two or more enantiomers; the eutomer is responsible for the therapeutic effect and the distomer for the unwanted- or even toxic side effects. Chiral drugs when administrated as racemate, may have many serious consequences. In order to minimize the problems associated with the distomer, pure enantiomers should be incorporated in the marketed medicines, and hence analysis methods must be developed to separate chiral pharmaceutical compounds. To facilitate chiral method development in any separation technique, a generic separation strategy can be applied in a short time at a relatively low cost. The strategy is usually composed of two steps; screening and optimization. These steps aim obtaining fast an idea about the enantioselectivity of the screened CSPs (chiral stationary phase or chiral selector), improving in the (quality of) separations and/or decreasing in analysis time (AT). The strategy should generic, i.e. it can be applied on structurally and chemically diverse compounds.
Capillary electrochromatography (CEC) is a hybrid technique that combines the advantages of both HPLC and CE. It has already proven its applicability in the field of chiral separation. Two chiral separation strategies were previously defined in CEC to screen acidic pharmaceutical compounds on the one hand and non-acidic (basic, amphoteric, neutral) on the other. These strategies used only one type of CSPs i.e. non-chlorinated polysaccharide-based CSPs. The strategies were updated by incorporating the chlorinated version of the CSPs. Using chlorinated CSPs in the CEC strategies for both non-acidic and acidic compounds resulted in improved success rates of both numbers: total and baseline separations, higher enantioselectivity and complementary results. Polysaccharide-based CSPs were chosen in the strategy because of their broad enantioselectivity.
In this poster presentation, an overview of the updated versions of the screening and optimization steps for both acidic and non-acidic pharmaceutical compounds in the CEC strategies was made and they were combined into one generic strategy. Recent modifications in both steps for the two strategies were highlighted. These strategies were evaluated and found applicable on structurally diverse molecules, showing their generic character.