|Abstract Title:||Chiral Stationary-Phase Optimized Selectivity Liquid Chromatography: a Novel Approach for the Separation of Mixtures of Enantiomers.|
|Session Choice:||Exploiting Separation Science|
|Presenter Name:||Mr Ravindra Hegade|
|Co-authors:||Dr Maarten De Beer|
Prof Frederic Lynen
Abstract Information :
The development of methods allowing improved chiral separation and purification of therapeutic agents is crucial as the pharmacokinetic and pharmacological behavior of both enantiomers and also of diastereomers can vary significantly from the active pharmaceutical ingredient. Recently Stationary-Phase Optimized Selectivity Liquid Chromatography (SOS-LC) has been successfully further developed and progressively used for isocratic1 and gradient2-4 liquid chromatography separations.5 Until now the potential of this methodology to facilitate the separation and purification of chiral isomers has not yet been investigated, which could speed up the purification process or allow for improved chiral screening of complex mixtures. In this work the possibilities of chiral Stationary-Phase Optimized Selectivity Liquid Chromatography (SOS-LC) are demonstrated on a limited set of commercial chiral columns. This approach allows for the prediction of the separation profiles through isocratic and gradient mode on any possible combination of the stationary phases based on a limited number of preliminary analyses. Isocratic and gradient predictions were performed with a commercially available and with an in-house developed Microsoft visual basic algorithm, respectively.
It has been shown that the retention time prediction on combined sets of commercial chiral columns is achievable with good accuracy. In both the isocratic and gradient work the predictive quality increased with the columns lengths, suggesting a somewhat higher sensitivity of the approaches in terms of injection processes. The potential of this method is demonstrated via the prediction of the optimal chiral column combination for the baseline separation of 4 chiral pairs containing solutes of varying polarity. The study also illustrates that the elution order of chiral pairs is not constant on all columns and that it appears to be highly dependent on the type of solute. The methodology offers perspective for faster analytical and preparative separation of optical isomers for various applications.