|A tutorial on the fundamentals of hydrophilic interaction chromatography (HILIC) for pharmaceutical analysis
|Fundamentals in Separation Science (KVCV)
|Dr James Heaton
Abstract Information :
HILIC can be a useful separation tool for the co-analysis of polar as well as ionisable pharmaceutical and related compounds. The HILIC modality can be used as an alternative or complementary technique to traditional reversed-phase chromatography (RPLC) approaches. HILIC can be a particularly suitable tool for the analysis of very polar compounds such as those with negative logP/logD values. As in RPLC, it is important to examine the composition of physiochemical properties and structural features of compounds in a given sample. In this tutorial we will explore the role of these features in directing HILIC method development work. Pertinently, the role of mobile phase buffer components and pH in conjunction with stationary phase chemistry will be discussed. Finally, best practice for successfully developing HILIC methods with relation to understanding compound structural features and diluent compatibility will be discussed.