WWEM - Abstract

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Abstract Title: Paper-based device for rapid and on-site wastewater surveillance
Presenter Name: Dr Zhugen Zhugen
Company/Organisation: Cranfield University
Country: United Kingdom

Abstract Information :

Wastewater-based epidemiology (WBE)has emerged as a powerful tool for early warning public health. However, it remains challenging for analysis of wastewater in particular for rapid and on-site detection, which is a highly demand for track down the infections. Rapid sensors are of significant importance for both chemicals (e.g. illicit drugs) and microbial analysis in wastewater (e.g. infection including SARS-CoV-2 for early warning of a pandemic). Here we present a low-cost, deployable paper-based biosensor device for rapid analysis of chemicals and pathogens for WBE. Following the demonstration of the paper-based device for field-testing of infectious in Indian local farm and for malaria testing in a primary school in Uganda in Africa, we will further show this device for the rapid analysis of pathogen in water and wastewater in a low resource setting (e.g., in quarantine hotel around London Heathrow airport). The device utilised a paper microfluidic for sample preparation and extract nucleic acid from various wastewater samples, followed by isothermal amplification for species-specific pathogen detection with a visual dye for rapid readout. This enables to capture images with a mobile phone camera for a semi-quantification. This device is currently developing to trace the source of SARS-CoV-2 in a local treatment plant and other near source tracking for early warning of the pandemic, within the frame of a UK national wastewater epidemiology surveillance programme (N-WESP) for COVID-19.

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ETP ILM Environment Agency RSC Group Water Forum.jpg RSC Group Science Forum.jpg Cogdem WMA Pump Centre SWIG Profilbus CIWEM Future Water Association Gambica WRC LPRA Zero Pollution Network WIPAC British Water Swan UKAS Scottish Environment Protection Agency Environment Protection Agency Natural Resources Wales