| Abstract Title: | The use of Short 10 mm Columns for Rapid LC-MS Analyses |
| Presenter Name: | Dr Arianne Soliven |
| Co-authors: | Mr Colin Pipe Mr David Dunthorne Mr Mark Fever Mrs Katie Lawlor Dr Lewis Couchman |
| Company/Organisation: | Avantor |
| Country: | United Kingdom |
Abstract Information :
Fast LC analysis is essential in high throughput laboratories, such as clinical, drug discovery and environmental labs. Over the last two decades, many labs have dramatically increased sample throughput using UHPLC, using shorter columns packed with sub-2 micron particles. However, the performance of modern mass spectrometers has continued to evolve. Improved sensitivity and ultra-fast data acquisition capabilities allow further reductions in analytical run times, using specially designed, high throughput columns. This presentation assesses the use of 10 mm columns for the analysis of complex samples, derived from biological or environmental sources. The theoretical considerations of short columns are discussed, starting with van Deemter theory, before moving to a kinetic plot interpretation of their application. Practical considerations of the use of such short columns will be discussed, specifically looking at dwell volumes, tubing and data acquisition rates. The analysis of complex samples requires a degree of sample preparation prior to application of high throughput LC-MSMS, due to matrix issues. The presentation will investigate the impact that the level of sample preparation has on the data integrity when applied to high throughput scenarios. Finally, the presentation will conclude with a series of applications detailing the benefits that the use of 10 mm length columns can have for high throughput analysis, without the loss of data integrity. The applications will include a series of therapeutic drugs, environmental pollutants and some samples from a hospital laboratory.
