SinS - Abstract

Abstract Title: Practical and accessible sustainable approaches for U/HPLC assays
Presenter Name: Dr Arianne Soliven
Co-authors:Dr Matt James
Prof Tony Edge
Mr Mark Fever
Company/Organisation: Avantor
Country: United Kingdom

Abstract Information :

The analytical laboratory may incorporate sustainable practices through their column selection decisions. From the analyst’s perspective, sustainable laboratory practices are tightly coupled to their consumption of solvents, reagents, and waste production. From the column manufacturers’ perspective, producing columns in smaller formats reduces the amount of stationary phase and stainless steel that is used. This talk focusses on the application of superficially porous particle (SPP) packed columns to drive more sustainable chromatography. Initially, the improvements in solvent consumption, throughput and efficiency that can be obtained by direct substitution of fully porous particles for equivalently sized SPP are demonstrated. We then move on to discuss how translating methodology to smaller format SPP columns, packed with smaller particle sizes can further improve the sustainability of chromatographic separations, demonstrating reductions in solvent consumption of up to 88%, alongside an 86% reduction in analysis time. The achievable savings are highlighted using a variety of pharmaceutical and peptide mapping assays, using column formats compatible with both HPLC and UHPLC instrumentation.