SinS - Abstract

Abstract Information :

The presentation aims to give an overview of how a liquid chromatography and mass spectrometry system (LCMS-8045 and Nexera X2, Shimadzu Corporation Europa GmbH, Milton Keynes, UK) has been successfully integrated into the biochemistry laboratory at the Carnegie School of Sport at Leeds Beckett University. It also provides an outline of the main concepts and goals of the overarching research project. The LCMS mass spectrometry systems have become an increasingly popular analytical tool in sports and nutritional science because of their sensitivity and specificity in identifying and quantifying compounds in complex biological matrices. Specifically, it can detect and quantify small molecules such as hormones, and several metabolites, making it a valuable tool in the analysis of sports supplements and nutritional interventions. Their use has also become widespread in doping control and forensic investigations in sports. With its capability to analyse a wide range of substances, such as nutrients, proteins, and drugs, the procurement of this equipment has enabled staff and PhD students at the Carnegie School of Sport at Leeds Beckett University to conduct analyses that support ongoing research and facilitate future aspirations. As the technology continues to advance, LCMS mass spectrometry is expected to play an even greater role in advancing our understanding of the effects of nutrition and exercise on human physiology. Furthermore, the presentation will provide a summary of two optimised methods for amino acid and steroid analyses and discuss the challenges encountered while implementing these methods. It will also provide a detailed description of the essential amino acid method, including the presentation of samples and standards, along with key findings of the completed projects. Through the optimisation of the analytical method for measuring amino acid concentrations, we were able to achieve rapid and precise quantification of 18 proteinogenic amino acids in plasma samples collected from older adults involved in research projects focusing on age-related sarcopenia. The samples were first filtered and labelled with a known quantity of standards to compensate for matrix effects and ensure accurate quantification. Chromatographic separation was performed using a specific column, and mass spectrometric detection was performed using an LCMS-8045 triple quadrupole instrument. A gradient elution was used, and the mobile phases were specified. Quality control samples were prepared to measure recovery and ensure the correct application of the method. Samples and standards were injected using an autosampler. In summary, the integration of LCMS technology in the Carnegie School of Sport has led to notable advancements in research quality and capacity. The instrument’s selection has enabled the acquisition of highly sensitive and intricate data. Our analysis of plasma amino acid levels has yielded promising findings, suggesting a possible association between decreased plasma amino acid concentrations and decreased muscle function in older adults. The implementation of this technology has the potential to foster the development of novel insights in our field.